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Novus Biologicals
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Bio-Techne corporation
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Proteintech
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Image Search Results
Journal: Molecules
Article Title: Strategic Fluorination to Achieve a Potent, Selective, Metabolically Stable, and Orally Bioavailable Inhibitor of CSNK2
doi: 10.3390/molecules29174158
Figure Lengend Snippet: In vivo CSNK2 inhibition. ( A ) SARS-CoV-2 viral titer measured in mouse lungs 24 h post-inoculation when treated with vehicle or with compound 2 (30 mg/kg p.o. b.i.d.) (n = 5). ( B ) AKT (phospho-Ser129) levels normalized to total AKT levels in mouse lungs following treatment with compound 2 (30 mg/kg p.o. b.i.d.) for 3, 6, and 36 h (n = 3). ( C ) EIF2S2 (phospho-Ser2) levels normalized to total EIF2S2 levels in mouse lungs following treatment with compound 2 (30 mg/kg p.o. b.i.d.) for 3, 6, and 36 h (n = 3). **: p = 0.0028 using unpaired t -test with Welch’s correction.
Article Snippet: Blots were blocked in 5% Milk in 1X TBST (0.1% Tween 20) for 1 h at room temperature, washed with 1X TBST, and incubated with primary antibodies in 5% BSA in 1X TBST for 10 h at room temperature; 1:10,000
Techniques: In Vivo, Inhibition
Journal: Journal of medicinal chemistry
Article Title: More than an Amide Bioisostere: Discovery of 1,2,4-Triazole-containing Pyrazolo[1,5- a ]pyrimidine Host CSNK2 Inhibitors for Combatting β-Coronavirus Replication.
doi: 10.1021/acs.jmedchem.4c00962
Figure Lengend Snippet: Figure 7. (A) Viral titer measured in mouse lungs 24 h post inoculation when treated with vehicle or with compound 53 (10 mg/kg i.p. b.i.d.) (n = 5). (B) Western blot for total EIF2S2 expression in mouse lung samples after treatment with vehicle or 53 (10 mg/kg i.p. b.i.d.) (n = 3). (C) Western blot for EIF2S2 (phospho-Ser2) in mouse lung samples after treatment with vehicle or 53 (10 mg/kg i.p. b.i.d.) (n = 3). (D) EIF2S2 (phospho-Ser2) levels normalized to total EIF2S2 levels in mouse lung. (E) Western blot for total AKT expression in mouse lung samples after treatment with vehicle or 53 (10 mg/kg i.p. b.i.d.) (n = 3). (F) Western blot for AKT (phospho-Ser129) in mouse lung samples after treatment with vehicle or 53 (10 mg/kg i.p. b.i.d.) (n = 3). (G) AKT (phospho-Ser129) levels normalized to total AKT levels in mouse lung.
Article Snippet: Blots were blocked in 5%Milk in 1X TBST (0.1% Tween 20) for 1 hour at room temperature, washed with 1X TBST, and incubated with primary antibodies in 5% BSA in 1X TBST for 10 hours at room temperature; 1:10,000
Techniques: Western Blot, Expressing